ANA Patterns

ANA Patterns - Cytoplasmic Patterns

Code	Pattern	Previous Nomenclature	Description

AC-15

Cytoplasmic fibrillar linear

actin-like

This pattern is characterized by decorated cytoskeletal fibers, sometimes with small, discontinuous granular deposits. Typical staining show striated actin cables spanning the long axis of the cells. e.g. anti-actin, anti-non-muscle myosin.

AC-16

Cytoplasmic fibrillar filamentous

none

Staining of microtubules and intermediate filaments spreading from the nuclear rim. e.g. anti-cytokeratin, anti-vimentin.

AC-17

Cytoplasmic fibrillar segmental

none

Enhanced decoration of short segments, periodic dense bodies, along the stress fibers. e.g. anti-alpha-actinin, anti-vinculin, anti-tropomyosin.

AC-18

Cytoplasmic discrete dots/GW body-like

GW body, processing body, lysosome*

Staining of GW bodies in the cytoplasm of interphase cells with high numbers in late S/G2 cells. e.g. anti-GW182, anti-Su/Ago2.

AC-19

Cytoplasmic dense fine speckled

homogeneous

The pattern appears cloudy, almost homogeneous throughout the cytoplasm. e.g. anti-PL-7.

AC-20

Cytoplasmic fine speckled

speckled

Scattered small speckles in the cytoplasm mostly with homogeneous or dense fine speckled background. e.g. anti-Jo-1.

AC-21

Cytoplasmic reticular/AMA

mitochondria-like

Coarse granular filamentous staining extending throughout the cytoplasm. e.g. anti-mitochondrial antibodies.

AC-22

Polar/Golgi-like

none

Discontinuous speckled or granular perinuclear ribbon-like staining with polar distribution in the cytoplasm. e.g. anti-giantin, anti-golgin-245.

AC-23

Rods and rings

none

Distinct rod and ring structures in the cytoplasm of interphase cells. Some smaller rods and rings also reported in the nucleus.